Abstract
Nuclear pore arrangement in synchronous cultures of Saccharomyces cerevisiae has been investigated using the freeze-fracture technique. In resting cells, the nucleus is small and located next to the large central vacuole. The relatively large area of nuclear surface adjacent to the vacuole appears to be indented by it and is entirely devoid of nuclear pores. During G0(lag phase) and the beginning of the S phase, the large vacuole undergoes progressive fragmentation into a number of smaller ones while the nucleus enlarges and assumes a more central position in the cell. Throughout this process, some vacuoles are observed in close association with the nucleus, causing an increasing number of progressively smaller pore-free depressions, thereby giving rise to a more uniform overall distribution of pores. These changes in the pore-free areas are consistent with an explanation in terms of lateral mobility of nuclear pore complexes in the nuclear envelope.
Published Version
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