Abstract
Malpighian tubules of Triatoma infestans cultivated in vitro in presence of 0.05 mM sodium valproate (VPA) for 1-4 h had their nuclear phenotypes investigated regarding induction of chromatin remodeling. Under present conditions, although a few nuclei demonstrated heterochromatin decondensation, no overall differences resulted in comparison to tests in which live insects are injected with VPA.
Highlights
Valproic acid/sodium valproate (VPA), an anticonvulsant widely prescribed for treatment of seizure disorders, is a well-known histone deacetylase inhibitor and a chromatin remodeling agent[1,2]
Considering the hypothesis that the drugs injection may not result totally effective to reach the cells in this work we examined the nuclear phenotypes of T. infestans Malpighian tubules cultivated in vitro in presence of VPA
Malpighian tubules removed from 5th instar nymphs of T. infestans (Klug) supplied by SUCEN (Mogi-Guaçu, SP) were cultivated for 1 to 4 h in the Leibovitz L-15 medium
Summary
Valproic acid/sodium valproate (VPA), an anticonvulsant widely prescribed for treatment of seizure disorders, is a well-known histone deacetylase inhibitor and a chromatin remodeling agent[1,2]. Nuclear phenotypes of Triatoma infestans Malpighian tubules cultivated in vitro in presence of sodium valproate Malpighian tubules of T. infestans cultivated in vitro in presence of 0.05 mM sodium valproate (VPA) for 1-4 h had their nuclear phenotypes investigated regarding induction of chromatin remodeling.
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