Abstract
The interaction of Ca~(2+) with sialic acid has been investigated by 500 MHz proton nuclear magnetic resonance spectroscopy. The changes of chemical shifts were observed for all sialic acid protons at different Ca~(2+) concentrations, and these spectral observations were also accompanied by changes in the J-coupling constants between the H_7 and H_8 protons and between the H_8 and H'_9 potens. Analysis of these Ca~(2+)-induced changes indicated that three oxygen atoms among (OH)_7, (OH)_8 and (OH)_9 of sialie acid formed a geometrical space suitable for Ca~(2+) coordination As a consequence, sialic acid provided a high-affinity binding site for Ca~(2+), the binding constant was about 104.72m~(-1).
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