Abstract
Nuclear magnetic resonance (NMR) micro-imaging and X-radiography were compared to assess their potential as non-destructive techniques for studying the germination of pepper ( Capsicum annuum L.) seeds. The radiography technique provided information about internal seed quality in dry seeds but yielded foggy images during their imbibition phase. In contrast, NMR micro-imaging clearly displayed the radicle, cotyledons and vascular system of the imbibed seeds. Dry seeds were selected from X-ray pictures and NMR images were recorded during their imbibition at a 1H frequency of 400 MHz with an in-plane resolution of 33 μm × 33 μm. The chemical shift difference between water and lipid proton resonances was exploited to monitor changes in the distribution of the two bulk fractions. The experiments showed the effectiveness of NMR micro-imaging in the study of the morphology of germinating seeds.
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