Abstract
Cultivation of Chlorella vulgaris Buitenzorg in a pilot scale of bubble column photo bioreactor using simulated NOx enriched flue gas concluded that presence of N 2 O as simulated NOx pollution (0.02%) in blowing bubbled air and CO 2 is not so significant, compare to control experiment that was designed by absence of N 2 O (around 20% decreased). Meanwhile, presence of N 2 O tends a less significantly decreasing of μ - specific growth rate and q CO 2 – specific CO 2 transferred rate. It is around 30% decreased in both of μ and q CO 2 . Then, cultivation by presence of NOx in blowing simulated flue gas could drastically decreased intracellular carotene and lipid content and become increase to level near to both of pigment and lipid content in control experiment. Furthermore, cultivation by presence of NOx in blowing simulated flue gas also could drastically exchange intracellular fatty acid content and it become dominated by 16:0 species. Finally, refreshing cellular growth product with re-cultivation by blowing fresh air, could be restored the fatty acid content nearly to beginning microbial fatty acid content. It was happened cause of converting hexadecanoate species to octadecanoate species and it was shown that oleate (18:1) was dominating species.
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