Abstract
Simple SummaryFeed efficiency strongly influences the productivity and profitability in modern swine farming enterprises. Residual feed intake (RFI) as a measure of feed efficiency has been extensively studied, which is defined as the differing value between observed feed intake and expected feed intake based on growth and backfat. There is a potential that the porcine vitronectin plays a role in RFI through affecting the energy metabolism within muscles. Using sequencing technology, we found that rich variations and amino acid changes existed in key regions of vitronectin. In addition, a difference in variant frequencies was observed between local Chinese breeds (Sujiang, Jiangqu-hai, and Jiaxing-black) and three well characterized breeds (American Duroc, Canadian Duroc, and Berkshire) derived from the overseas. We suggest that the porcine vitronectin gene can be studied further and may be developed as a gene marker to select high feed-efficiency swines.Vitronectin plays a role in the blood homeostasis and has been implicated in cell adhesion, migration, and proliferation. Vitronectin has a potential role affecting the residual feed intake (RFI) or feeding efficiency in swine production. Its variations have not been reported in Chinese swine breeds. In this study, two regions of porcine vitronectin were analyzed using PCR and sequencing. The sequence analysis revealed thirteen nucleotide substitutions in region 1 (exon 2- exon 3) and three nucleotide substitutions in region 2 (exon 5- intron 5), which would result in five amino acid changes (p.Ala52Thr, p.Leu94Pro, p.Leu94Gln, p.Gln94Pro, and p.Glu126Gly). In region 1, c.156C/T, c.281A/T, and c.377A/G were the most common (at a total frequency of 49.3%, 31.3% and 31.9% respectively), whereas c.153C/T and c.180C/G were rare (at a total frequency of 1.39%). In region 2, c.597 + 12A/G was the most common (at a total frequency of 39.6%), followed by c.597 + 15A/G (at a total frequency of 31.3%) and c.459A/G (at a total frequency of 16.0%). There was a difference (p < 0.05) in variant frequencies between Chinese breeds and overseas breeds. These results indicate that the porcine vitronectin gene is polymorphic and suggest further analysis is required to see if the variation detected affects RFI or feed efficiency in swines.
Highlights
Vitronectin, abbreviated as VTN, is known as s-protein or serum diffusion factor
The porcine vitronectin gene is located in the genome region (g.44650622–g.44653871) of chromosome 12, including eight exons interrupted by seven introns
It is notable that thirteen nucleotide substitutions are novel and several substitutions in these positions (c.154G/A, c.281T/C, c.281A/T, c.281A/C, and c.377A/G) would result in amino acid changes (Table 3)
Summary
Vitronectin, abbreviated as VTN, is known as s-protein or serum diffusion factor. It is a multifunctional glycoprotein [1,2]. Animals 2019, 9, 520 was observed in the urethra and retina tissues [4,5]. It functions as a facilitator of cell adhesion, migration, and proliferation [6]. The porcine vitronectin gene is located in the genome region (g.44650622–g.44653871) of chromosome 12, including eight exons interrupted by seven introns. The single nucleotide substitutions have been reported in several above species
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
