Abstract
Recent taxonomic and molecular studies on Tuber in North America have revealed several new species. Here we propose Tuber bonitoi, T. brunneum, T. pseudoseparans and T. tequilanum as new species. These species are described from Mexico and differ in a combination of morphologically features including ascospore size, pellis cell size, peridium thickness and ascoma color. These new taxa have phylogenetic affinity to Tuber separans, described from the United States. Phylogenetic placement of these novel Tuber species in the /puberulum clade are presented
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