Abstract
Recently, the biosynthesis of nanoparticles has been well explored which draws attention to its possible biomedical applications especially in cancer therapy. In the current study, the novelty in the biosynthesis of silver nanoparticles (AgNPs) using honey bee extract has been explained. This study was also aiming at investigating the anti-colon cancer activities of the biogenic AgNPs along with its capping biomolecules in vitro. The obtained biogenic AgNPs were well characterized by X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). It was found that the formed AgNPs have spherical shape with size range from 12 to 18 nm embedded in honey bee biomolecules. The cytotoxicity results of AgNPs on peripheral blood mononuclear cells (PBMC) indicated that the obtained AgNPs could be used safely with concentrations upto 39 μg/ml. On the other hand, the potentialities of the biogenic AgNPs against colon cancer proliferation recorded 60% inhibition using its nontoxic dose with a down regulation of the expression of Bcl2 and survivin gene. By the extraction of AgNPs capping biomolecules to explain the exact fraction that is responsible for the anticancer properties, it was found that both AgNPs and its capping biomolecules have anti-proliferative effects with a priority to the naked AgNPs.
Highlights
Nanomedicine refers to the use of precision-engineered nanomaterials in order to reveal novel therapeutic and diagnostic tools for human use [1]
The formation of the AgNPs was confirmed through the color change in the reaction mixture (AgNO3 solution + honey bee extract) which is due to the reduction of the Ag+ ions into Ag atoms into nanoparticles
This absorption is attributed to the surface plasmon resonance (SPR) of the nanoparticles
Summary
Nanomedicine refers to the use of precision-engineered nanomaterials in order to reveal novel therapeutic and diagnostic tools for human use [1]. Among different nanoparticles investigated, AgNPs are the most promising particles that are used in the field of nanomedicine for their antimicrobial activity against different microbes [1]. There are limited studies on the potential of biogenic AgNPs as anticancer agents. Among these studies, there is a recent report on the anti-proliferative activities of AgNPs against human glioblastoma cells (U251) [2]. This report introduces a simple, cost-effective and environmentally benign synthesis of AgNPs using honey bee. The activity of the synthesized AgNPs against human epithelial colorectaladeno carcinoma was evaluated with referring to its molecular action on cellular gene expressions
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