Abstract
Phosphorylation of proteins involved in the regulation of contractile strength and relaxation speed are critical to cardiac output regulation. This phosphorylation happens very fast; in the fight-fright-flight response, phosphorylation occurs in mere seconds and can dissipate in tens of seconds. Likewise, in heart-rate dependent regulation of contractility, phosphoproteins are also prominently involved. In order to study phosphorylation levels in human tissue, tissue needs to be collected ideally under a well-controlled physiological state.
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