Abstract

Summaryhaploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter‐ and intraspecific via chromosome elimination. Here, we focus on haploidization via chromosome elimination, especially the recent advances in centromere‐mediated haploidization. Once haploids have been induced, genome doubling is needed to produce DH lines. This study has proposed a new strategy to improve haploid genome doubling by combing haploids and minichromosome technology. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis.

Highlights

  • Based on the 2015 Revision of World Population Prospects, the world population will reach 9.7 billion in 2050

  • Doubled haploid technology has been successfully used in crop improvement and genetic analysis

  • Conservation of MTL in cereals may enable the development of intraspecific in vivo haploid inducer lines in crop plants to accelerate plant breeding

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Summary

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Follow this and additional works at: http://lib.dr.iastate.edu/agron_pubs Part of the Agronomy and Crop Sciences Commons. The complete bibliographic information for this item can be found at http://lib.dr.iastate.edu/ agron_pubs/300. Abstract haploid inducer line can be transferred (DH) technology can shorten the breeding process and increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Once haploids have been induced, genome doubling is needed to produce DH lines. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis. Disciplines Agronomy and Crop Sciences Comments This article is published as Ren, Jiaojiao, Penghao Wu, Benjamin Trampe, Xiaolong Tian, Shaojiang Chen, and Thomas Lübberstedt. This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/agron_pubs/300

Review article
Introduction
Haploidization via interspecific hybridization
Arabidopsis Maize Maize Maize
Haploidization via intraspecific hybridization
Haploid ǀ
Haploid with introgression ǀ
Artificial genome doubling
Spontaneous genome doubling
Combing haploids with minichromosomes
Exchanging cytoplasmic and nuclear genomes
Reverse breeding
Gene stacking from biparental crosses
Accelerate plant breeding by MAS and GS
Findings
Summary and outlook
Full Text
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