Novel Synthesis of Oxidoreductase Immobilized Biocatalyst for Effective Anthraquinone Dye Treatment in Bioreactor

Abstract

Over the past few decades, biotransformations of compounds with complicated and reinforced structures, especially some poorly biodegradable organic pollutants, have attracted extensive research attention. Benefiting from recently developed techniques of protein engineering, oxidoreductase industrial applications such as laccases, tyrosinases, and various oxygenases have been recognized as a promising alternative technique as compared with the conventional treatment processes of industrial textile effluents. However, the lack of long-term operational stability and reusability of the above-mentioned enzymes may limit their further large-scale industrialization. To overcome this, a novel biocatalyst was developed by immobilizing laccase from Trametes versicolor onto ultraporous gamma-alumina powders (laccase@UPA(γ)), followed by transferring it into a portable and easy-to-carry bioreactor for Remazol Brilliant Blue R (RBBR) dye biodegradation. The obtained results showed that the treatment capacity of laccase@UPA(γ) towards RBBR reached about 60 mg/g after 24 h of contact time at pH 5. These preliminary results highlight the potentials of bio-based inorganic materials in industrial wastewater treatment, which can broaden our understanding of their practical applications in the environmental field.