Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class

Abstract

One of the ways to increase the specificity and biocompatibility of cytostatic drugs is the creation of conjugates of drugs with graphene oxide. Graphene and its oxidized form - graphene oxide (GO) - have become new promising materials in the field of nanomedicine due to its reactive and developed surface that can be subjected to covalent and non-covalent functionalization, which allows provide the immobilization of drugs. This article is devoted to a new method for the synthesis, identification and study of the biocompatibility of a non-covalent conjugate based on GO and an alkylating agent based on 1,3,5-triazine. The conjugates are hemocompatible (the degree of hemolysis does not exceed 5 % in the concentration range 1–200 mg∙l−1), exhibit antioxidant activity in the model reaction with DPPH, Radachlorin (the photodegradation constant decreased by 1.7 times at the maximum concentration of GO-1 75 mg∙l−1) and photoinduced hemolysis, has dose-dependent genotoxicity, and also exhibit cytotoxicity towards cell lines A549, PANC-1 and HeLa. The maximum cytotoxicity is shown in the HeLa cell line (IC50 = 2.5 µM).