Novel molecular diagnosis of Cyl E, Spb 1 and bib A virulence genes of Streptococcus agalactiae from pregnant women

Abstract

Group B Streptococcus is a gram-positive coccal bacterium which produces an arrow zone of blood hemolysis on blood agar. Group B Streptococcus is the most common cause of invasive bacterial illness in neonates, including sepsis, meningitis, septicemia, and pneumonia. For the first time, such a study of Streptococcus agalactiae has been conducted in hospitals of Anbar Governorate .All Streptococcus agalactiae identified by conventianal methods , automated methods using Vitek-2 system , and molecular methods by conventional PCR. All isolates gave a positive result as 16S rRNA. Distribution of resistance ratio for Streptococcus agalactiae were Penicillin 20% , Clindamycin 68% , Chloramphenicol % 46 , Tetracycline 82% , Erythromycin % 100 ,Imipenem % 12 , and Ciprofloxacillin 26.47%. The Cyl E and bibA genes were detected by conventional PCR and the result showed (72%) strains positive, but in case of bla Cyl E gene (53.48%) of strains harbored bla bibA genes , while 48.83 of Streptococcus agalactiae contain Spb1 gene . This approach showed the coexistence of bla Cyl E , Spb 1 and bibA genes in 48.83% strains of Streptococcus agalactiae.