Abstract
Objective The aim of this study was to evaluate the effect of reuterin produced by a novel probiotic strain of Lactobacillus reuteri against periodontal biofilms. Materials and Methods L. reuteri LC382415 (an indigenous Indonesian strain) was cultured in Man, Rogosa, and Sharpe (MRS) agar in anaerobic conditions for 24 hours. To isolate reuterin, L. reuteri was suspended in 300-mM glycerol in MRS broth and incubated under anaerobic conditions for 3 hours, and the supernatant fraction was filtered. The presence of reuterin was confirmed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and its concentration was determined. The effect of reuterin on Porphyromonas gingivalis ATCC 33277 and T. denticola ATCC 35405 biofilms was evaluated using biofilm assays. Biofilms were formed by incubating bacteria in 96-well microplates for 48 hours. A dose-dependent experiment was performed with reuterin concentrations of 12.5, 25, 50, and 100 μg/mL on biofilms. The inhibitory effect was measured at 1, 3, 6, and 24 hours. The biofilm masses were measured at 490 nm. Statistical analysis was using one-way ANOVA. Results The SDS-PAGE assay confirmed the presence of reuterin (52 kDa) in the culture supernatant of the L. reuteri strain. Reuterin in a concentration as low as 12.5 μg/mL significantly inhibited single- and mixed-species biofilms ( p < 0.05). Conclusions This is the first study to demonstrate the promising effect of reuterin isolated from L. reuteri LC382415 against periodontal bacteria. Further studies are warranted to explore the mechanism of this active component.
Highlights
Periodontitis is considered one of the most prevalent diseases worldwide and the main cause of adult tooth loss.[1]
Isolation of Reuterin, SDS-PAGE, and Bradford Assay The presence of reuterin isolated from L. reuteri LC382415 and ATCC 55730 is shown in ►Fig. 1
Activity of Reuterin against Pathogenic Periodontal Biofilms The photographs of the biofilm lining 96-well plates of reuterin against each monospecies P. gingivalis and T. denticola were taken after staining with crystal violet
Summary
Periodontitis is considered one of the most prevalent diseases worldwide and the main cause of adult tooth loss.[1] It is associated with systemic pathologies, such as cardiovascular disease, obesity, and diabetes.[2] The etiologic agents of periodontal disease are pathogenic dental plaque biofilms.[3] Under certain conditions, these biofilms harbor gram-negative bacterial pathogens such as Porphyromonas gingivalis and Treponema denticola, which are closely associated with periodontitis.[4] These bacteria produce virulence factors that evoke an exacerbated immune-inflammatory response, leading to the destruction of periodontal tissues. Periodontitis is treated by mechanical debridement of dental plaque combined with chemical plaque control measures.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
