Novel Globular Polymeric Supports for Membrane-Enhanced Peptide Synthesis

Abstract

Membrane-enhanced peptide synthesis (MEPS), a technique that combines liquid-phase peptide synthesis (LPPS) with organic solvent nanofiltration (OSN), has emerged as a new methodology to tackle current challenges in solid-phase peptide synthesis (SPPS), the current strategy of choice for the preparation of peptides. This new technology platform is scalable beyond kilogram scale, automatable, and compatible with established Fmoc chemistry, as it combines chemistry in solution with expedient membrane purification. Here we screened novel highly rejected soluble polymeric supports and studied their application for the preparation of a model peptide. Our findings make a significant contribution to the development of MEPS.