Abstract
ABSTRACTA new electrochemical immunoassay based on a sandwich format was developed for carcinoembryonic antigen using ferrocenyl polymer nanospheres as the recognition elements. Ferrocenyl nanospheres were synthesized by coordination polymerization and characterized by transmission electron microscopy, energy dispersive X-ray spectrometry, and infrared spectroscopy. Monoclonal carcinoembryonic antibodies were immobilized on a nanogold-modified gold electrode to enhance the sensitivity. Polyclonal carcinoembryonic antibodies were conjugated on ferrocenyl polymer nanospheres. Under the optimal conditions, the electrochemical signal increased with the concentration of the analyte from 0.01 to 50 ng mL−1 with a limit of detection equal to 0.004 ng mL−1. The immunosensor provided acceptable reproducibility with coefficients of variation below 10%.
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