Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis

Abstract

ABSTRACTCapillary electrophoresis has become an effective tool for the separation and analysis of biomolecule macromolecule.In this work, a new method to prepare covalent connection capillary electrophoretic coatings is proposed. Photosensitive diazoresin (DR) as coupling agents and carboxymethyl chitosan as coating was used. Through layer by layer (LBL) self-assembly and unique photochemistry reaction of DR, a stable and efficient capillary coating can be obtained. The covalently bonded coatings have the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus a baseline separation of Lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be achieved. The covalently linked DR/carboxymethyl chitosan capillary coatings presented good stability and repeatability. This method seems to be an environmental and simple way to prepare the covalently coated capillaries for CE.