Novel combination of multi-walled carbon nanotubes and gold nanocomposite for photothermal therapy in human breast cancer model

Abstract

Despite current medical advancements, the resistance of malignant tumours to conventional medical therapies highlights the need for innovative therapeutic techniques. Numerous studies have focused on the promising application of nanomaterials in recent years. Nanoparticles (NPs) are used to treat cancer. Plasmonic photothermal therapy (PPTT) is a cancer-ablation technique in which photon energy is rapidly converted into heat by some radiative and non-radiative events. Gold NPs (Au-NPs) and carbon nanotubes (CNTs) are plasmonic NPs with excellent thermal conductivity and their near-infrared (NIR) absorbance has several interesting qualities. Additionally, CNTs could penetrate cells. In this study, Au-NPs were used to fabricate multi-walled CNTs (MWCNTs), which could boost its efficacy in cancer treatment in accordance with PPTT. Transmission electron microscopy, field-emission scanning electron microscopy (FESEM), atomic force microscopy and FTIR were used to examine the MWCNTs made from walnut shell. Au-NPs were explored using green chemistry and MWCNT-COOAu, MWCNT-COO and MWCNT-Au were examined by Raman, EDX and FESEM techniques. The effect of MWCNT-COOAu, MWCNT-COO and MWCNT-Au at various concentrations (3.12, 6.25, 12.5 and 25 µg/mL) and irradiation time intervals (30, 60, 90 and 120 sec) by using NIR laser under λ = 1064 nm and P = 3 W on the breast cancer cell line (MCF7) was investigated. The highest temperatures for MWCNT-COO, MWCNT-COOAu and MWCNT-Au were determined to be 44.1 °C, 46 °C and 46.9 °C, respectively, which produced 61.66 %, 72 % and 85.3 % cytotoxicity, respectively, in MCF7 cell line at a concentration of 25 µg/mL and an irradiation period of 120 sec. The treatment of MCF7 cell line by photothermal therapy was found to be in a concentration- and time-dependent manner.