Abstract
Novel biosynthesis of monogalactosyl-alkylacyl glycerolipid in Mop8 fibroblast cells transfected with a ceramide galactosyltransferase gene
Highlights
Cer is a major bioactive lipid in eukaryotic cells and plays the role of a lipid second messenger in cell signaling transductions [1,2]
We speculated that accumulation of Cer under heat stress might be a trigger to activate the synthesis of monohexosylceramides (GlcCer and/or GalCer) to decrease the intracellular Cer level and evade cell apoptosis
We confirmed that the expression of the GlcT gene in Mop8 fibroblasts was activated in a temperature-dependent manner under heat stress [8]
Summary
Cer is a major bioactive lipid in eukaryotic cells and plays the role of a lipid second messenger in cell signaling transductions [1,2]. It is well known that various stresses such as high temperature and ultraviolet irradiation elevate intracellular Cer levels followed by cell apoptosis [4]. In previous studies we demonstrated that hyperosmotic stress to MDCK cells increased Cer content, and the mRNA level of GalT was upregulated resulting in GalCer accumulation [5]. In our recent study heat-stressed MDCK cells had an increased content of Cer, and the de novo synthesis from serine and both GlcCer and GalCer syntheses from Cer were shown to increase by metabolic labeling [7]. We speculated that accumulation of Cer under heat stress might be a trigger to activate the synthesis of monohexosylceramides (GlcCer and/or GalCer) to decrease the intracellular Cer level and evade cell apoptosis. The Cer metabolism in this transfectant, molecular species of the newly expressed glycosyl glycerolipid characterized with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) with high energy collision-induced dissociation (CID) are discussed
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