Abstract
Note on the phylogenetic position of Eptesicus species in the Vespertilionidae family (Chiroptera) using Brazilian bats and RAG2 sequences.
Highlights
It is clear that the Vespertilionidae family still has phylogenetic issues to be cleared out, specially regarding the positioning of Eptesicus species within the genus and its relation with the Histiotus genus
Based on the Akaike information criterion (Posada and Buckley, 2004), the dataset was evaluated for the appropriate model of molecular evolution using ModelTest v.3.7 (Posada and Crandall, 1998). Clade support for both maximum parsimony (MP) and maximum likelihood (ML) phylogenies were assessed with a bootstrap analysis using 1,000 replicates with 100 random addition sequence replicates, and tree bisection and reconnection (TBR) branch swapping as implemented in PAUP* 4.01b10 (Swofford, 2002) using the interface of Geneious® 8.0.2
These results were not observed by Giménez et al (2019), which observed a weak support for this separation using one nuclear and one mitochondrial gene, mostly because of the incongruence of ML with the Bayesian inference (BI) and MP analyses
Summary
It is clear that the Vespertilionidae family still has phylogenetic issues to be cleared out, specially regarding the positioning of Eptesicus species within the genus and its relation with the Histiotus genus. Complementary DNA sequences were assembled for each accession using Geneious® 8.0.2 (Biomatters, 2015). Multiple-sequence alignment was performed using Clustal W (Chenna et al, 2003) followed by manual optimization. The resolution and support were affected in substantial way by inclusion of gaps, with gaps treated as fifth base (results shown).
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