Abstract
To investigate the role of norathyriol in enhancing the endothelial monolayer paracellular barrier function, and preventing disturbance of the paracellular barrier function by vasoactive agents, this study examined the effect of norathyriol on serotonin-mediated macromolecule transfer and microfilament change in cultured rat heart endothelial cells (RHEC). Incubation of the endothelial monolayer with norathyriol antagonized serotonin-and cytochalasin B-induced macromolecular permeability. Norathyriol also opposed the effect of serotonin on the distribution of actin filaments in the endothelial cytoskeleton. Upon addition of 10^(-6) M norathyriol, serotonininduced changes in the myosin and distribution of myosin were comparable to that of serotonin monolayers. In conclusion, norathyriol decreased the permeability of RHEC caused by serotonin, which was associated with the stabilization of F-actin microfilaments and changes in the myosin organization, suggesting that influences on cytoskeletal assembly may be involved in this process.
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