Non-volatile disinfection byproducts are far more toxic to mammalian cells than volatile byproducts

Abstract

Water is often chlorinated to protect public health, but chlorination causes harmful disinfection byproducts to form. Currently available in vitro assays generally determine non-volatile disinfection byproduct (NVDBP) toxicities because of the limitation of pretreatments used, but chemical analyses and regulations are focused on volatile disinfection byproducts (VDBPs) such as trihalomethanes. The gap of VDBP toxicities have been of concern for some time. In this study, we extracted VDBPs from two chlorinated effluent organic matters and one chlorinated natural organic matter, using a helium aeration–liquid nitrogen condensation system, and systematically assessed the VDBP and NVDBP toxicities to mammalian cells. VDBPs accounted for 10%–20% of the total organic halogen concentrations in three chlorinated water samples. VDBPs were much less cytotoxic, caused fewer DNA double-strand breaks, induced less reactive oxygen species and DNA/RNA oxidative damage marker of 8-hydroxyl(deoxy)guanosine in cells than did NVDBPs. Moreover, by collecting the VDBPs, toxicity measurement of the full range of DBPs was achieved. Cytotoxicity, reactive oxygen species and 8-hydroxyl(deoxy)guanosine levels were significantly higher for cells exposed to the mixture of VDBPs and NVDBPs than only NVDBPs, but not by large percentages (20%–30% for cytotoxicity), suggesting NVDBPs mainly contributed to the toxicity of chlorinated water. Our study suggested that future research should focus more on NVDBP toxicity and identifying toxicity drivers from NVDBPs.