Abstract
Non-thermal plasma (NTP) has many functional activities such as, sterilization, wound healing and anti-cancer activity. Despite of its wide spread biomedical application, the effect of NTP on immune cells and allergic response has not been well studied. In this study, we determined whether NTP suppresses mast cell activation, which is important for allergic response, and ameliorates an atopic dermatitis (AD)-like skin inflammatory disease in mice. Exposure to NTP-treated medium during mast cell activation inhibited the expression and production of IL-6, TNF-α and suppressed NF-κB activation. We also investigated whether NTP treatment ameliorates house dust mite (HDM)-induced AD-like skin inflammation in mice. NTP treatment inhibited increases in epidermal thickness and recruitment of mast cells and eosinophils, which are important cell types in AD pathogenesis. In addition, Th2 cell differentiation was induced by application of HDM and the differentiation was also inhibited in the draining lymph node of NTP-treated mice. Finally, the expression of AD-related cytokines and chemokines was also decreased in NTP-treated mice. Taken together, these results suggest that NTP might be useful in the treatment of allergic skin diseases, such as AD.
Highlights
Atopic dermatitis (AD) is a common allergic skin disease, characterized by mast cell activation, eosinophilia, overexpression of cytokines and epithelial hyperplasia[1,2]
We investigated whether non-thermal plasma (NTP) inhibited mast cell activation and house dust mite (HDM)-induced atopic dermatitis (AD)-like skin inflammation in NC/Nga mice
Our results show that NTP treatment inhibited HDM-induced AD-like skin inflammation in NC/Nga mice
Summary
Atopic dermatitis (AD) is a common allergic skin disease, characterized by mast cell activation, eosinophilia, overexpression of cytokines and epithelial hyperplasia[1,2]. Several treatments for AD, such as glucocorticoids, calcineurin inhibitors, phototherapy, and immunosuppressors (cyclosporine A), have been used[8] These drugs and therapies cause many side effects including ulcers, thin skin, diabetes, depression, and slow-wound healing[9]. We demonstrated that non-thermal plasma (NTP) treatment induces cancer cell death via AKT degradation[17], promotes muscle regeneration in mice[18], accelerates wound healing[19], and inhibits psoriasis-like skin inflammation in mice[20]. We investigated whether NTP inhibited mast cell activation and house dust mite (HDM)-induced AD-like skin inflammation in NC/Nga mice. Www.nature.com/scientificreports plasma (LTP) on mast cell activation and the inflammation of keratinocytes, suggesting that the anti-allergic effect of NTP might at least partially result from the suppression of mast cell and keratinocyte activation
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