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蛍光分子の2光子励起に代表される非線形光学現象には，それ以外にも様々な種類がある．これらは生細胞イメージングに適した特長を共有しつつ，それぞれ独自の情報を観測者に与える．近年の技術発展により応用性が拡大する種々の非線形光学顕微鏡の活用により，生細胞の可視化解析に新たな展開がもたらされると期待される．
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