Abstract
BackgroundCerebral blood flow (CBF) quantitation using technetium-99m hexamethylpropyleneamine oxime (99mTc-HMPAO) generally requires assessment of input function by arterial blood sampling, which would be invasive for small animals. We therefore performed chest dynamic planar imaging, instead of arterial blood sampling, to estimate the input function and establish noninvasive quantitation method of rat CBF using the image-derived input function.ResultsIntegrated radioactivity concentration in the heart-blood pool on planar images (AUCBlood-planar) was identical to that in arterial blood samples (AUCBlood-sampling). Radioactivity concentration in the brain determined by SPECT imaging (CBrain-SPECT) was identical to that using brain sampling (CBrain-sampling). Noninvasively calculated CBF obtained by dividing CBrain-SPECT by AUCBlood-planar was well correlated with conventionally estimated CBF obtained by dividing CBrain-sampling by AUCBlood-sampling.ConclusionRat CBF could be noninvasively quantitated using 99mTc-HMPAO chest dynamic planar imaging and head SPECT imaging without arterial blood sampling.
Highlights
Cerebral blood flow (CBF) quantitation using technetium-99m hexamethylpropyleneamine oxime (99mTc-HMPAO) generally requires assessment of input function by arterial blood sampling, which would be invasive for small animals
Input function assessed by dynamic imaging of a large blood pool is used for quantitative analysis of some single-photon emission computed tomography (SPECT) and positron emission tomography (PET) radiotracers in humans [8,9,10] and small rodents [11]
Rat CBF was quantified using the input function obtained from dynamic planar images and compared with the CBF conventionally quantified using arterial blood sampling
Summary
Cerebral blood flow (CBF) quantitation using technetium-99m hexamethylpropyleneamine oxime (99mTc-HMPAO) generally requires assessment of input function by arterial blood sampling, which would be invasive for small animals. We performed chest dynamic planar imaging, instead of arterial blood sampling, to estimate the input function and establish noninvasive quantitation method of rat CBF using the image-derived input function. Input function assessed by dynamic imaging of a large blood pool is used for quantitative analysis of some SPECT and positron emission tomography (PET) radiotracers in humans [8,9,10] and small rodents [11]. We performed dynamic planar imaging of the heart after intravenous administration of 99mTc-HMPAO to assess image-derived input function. Rat CBF was quantified using the input function obtained from dynamic planar images and compared with the CBF conventionally quantified using arterial blood sampling
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