Abstract
Analytical techniques used to extract chlorophyll from plant leaves are destructive and based on the use of organic solvents. This study proposes a non-destructive quantification of the photosynthetic pigment concentration in cotton leaves using two portable chlorophyll meters, the SPAD-502 and the CLOROFILOG 1030. After obtaining 200 leaf discs, each with an area of 113 mm2, the greening rate in each disc was determined by the average of five readings from both meters. Immediately after measurement, 5 mL of dimethyl sulfoxide (DMSO) was added, and the samples were kept in a water bath at 70ºC for 30 min. After cooling, 3 mL of the liquid extract was used for analyses by spectrophotometry at 470, 646 and 663 nm. Mathematical models were adjusted from analytical results using the reading index obtained from both devices to predict the contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids. Based on these results, it was concluded that both portable chlorophyll meters are an effective way to estimate the concentration of photosynthetic pigments in cotton leaves, thus saving time, space and the resources that are often required for these analyses.
Highlights
There are important factors related to photosynthetic efficiency in plants, such as the concentration and composition of chloroplast pigments that affect plant growth and their adaptability to environments with different luminosities (DAI et al, 2009).The production of dry matter by crop species and their ability for abiotic stress tolerance has been influenced by the amount of chlorophyll (Chl) present, due to the vital relationship of this pigmentActa Scientiarum
Losses in chlorophyll content are associated with damaging environmental factors, such that variations in the total chlorophyll/carotenoids ratio are good indicators of plant injury (HENDRY; PRICE, 1993; KARA; MUJDECI, 2010)
The main objectives of this study were the following: to assess the cotton chlorophyll composition and establish a possible correlation between the photosynthetic pigments extracted in dimethyl sulfoxide (DMSO) with readings obtained by both chlorophyll meters; and to verify the relationship between these characteristics in the leaf tissue of Upland Cotton cultivated under field conditions
Summary
There are important factors related to photosynthetic efficiency in plants, such as the concentration and composition of chloroplast pigments that affect plant growth and their adaptability to environments with different luminosities (DAI et al, 2009).The production of dry matter by crop species and their ability for abiotic stress tolerance has been influenced by the amount of chlorophyll (Chl) present, due to the vital relationship of this pigmentActa Scientiarum. There are important factors related to photosynthetic efficiency in plants, such as the concentration and composition of chloroplast pigments that affect plant growth and their adaptability to environments with different luminosities (DAI et al, 2009). The production of dry matter by crop species and their ability for abiotic stress tolerance has been influenced by the amount of chlorophyll (Chl) present, due to the vital relationship of this pigment. Losses in chlorophyll content are associated with damaging environmental factors, such that variations in the total chlorophyll/carotenoids ratio are good indicators of plant injury (HENDRY; PRICE, 1993; KARA; MUJDECI, 2010). The determination of the leaf chlorophyll content is a common procedure for plant scientists. Destructive techniques have been traditionally used for the determination of chlorophyll content in stands of vegetation.
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