Abstract
These data demonstrate that the phenomenon of non-vectorial phosphorylation [Saier, M.H. jr and Schmidt, M.R. (1981) J. Bacteriol. 145, 391–397] is an experimental artifact which arises during the conversion of whole cells to spheroplasts or membrane vesicles. It can be explained by a certain population of particles which are not properly sealed. There is no need to envoke two orientations of enzyme II in the membrane, one of which would have no physiological significance.
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