Abstract
This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (n = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (p < 0.05), without interactions with EDTA (p > 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.
Highlights
The purpose of this study was to investigate the effects of Non-Thermal Atmospheric Pressure Plasma (NTAPP) treatment of human root dentin surfaces in the recruitment, attachment and growth of human dental pulp stem cells (hDPSCs)
Dentin slices were randomly assigned (n = 9/group) for conditioning as follows: Group I, NaOCl (1.5%, 10 mL/5 min), ethylenediaminetetraacetic acid (EDTA) (17%, 10 mL/5 min), followed by NTAPP; Group II, NaOCl, NTAPP; Group III, NaOCl
Dentin slices were randomly assigned (n = 9/group) for conditioning as follows: Group I, NaOCl (1.5%, 10 mL/5 min), EDTA (17%, 10 mL/5 min), followed by NTAPP; Group II, NaOCl, NTAPP; Group III, NaOCl, EDTA; Group IV, NaOCl alone
Summary
Non-thermal atmospheric pressure plasma (NTAPP) involves partially ionized gas containing electrically charged particles at atmospheric pressure. These include reactive oxygen and nitrogen species that induce various physiological effects, with promising clinical and preclinical applications in plasma medicine. Recent developments include direct and indirect applications of NTAPP for microbial biofilm eradication, tissue disinfection, wound healing, tissue rejuvenation, and surface modification of biological scaffolds. NTAPP has been studied for periodontal pocket decontamination, adhesion, caries treatment, root canal disinfection, implant surface treatment, and tooth bleaching [1,2]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
