Abstract
Only parts of heavy metal ions have sensitive and selective deoxyribozymes(DNAzymes), but most of heavy metal ions do not have the specific DNAzyme that limits the development of high-precise biosensors. As for cadmium ion (Cd2+), it lacks a specific DNAzyme that can be interfered by the multiple heavy metal ions (Hg2+ and Pb2+), causing poor accuracy if only non-specific DNAzyme-based biosensor measures the Cd2+ concentration. Here, one approach to solve this problem is proposed that uses in conjunction with other biosensors and the analysis data model to explore the interference law and improve the detection accuracy. The non-specific DNAzyme-based biosensor is fabricated using a field-effect transistor(FET), single-walled carbon nanotube(SWNTs) and non-specific DNAzyme. The performances are characterized by different spectral and electronic methods, indicating excellent sensitivity, stability and repeatability. Under the optimized conditions, the non-specific DNAzyme-based biosensor can determine Cd2+ down to 3.4 × 10-2 nM, and the linear range is varying from 5.0 × 10-2 to 1.0 × 103 nM with the correlation coefficient 0.98. To qualitatively and quantitatively analyze Cd2+ in a real sample, other biosensors with high sensitivity for Hg2+ and Pb2+ are selected and optimized to form a biosensor array, and then the detecting signals are processed by a mathematical model. At last, the prepared biosensor array is applied to measure the Cd2+ in feed to evaluate the detection accuracy.
Published Version
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