Abstract
Mixing techniques, which kept killed or non-multiplying Tetrahymena pyriformis in fully randomized suspensions for short test periods (hours), were inadequate for ideal mixing of normal, untreated cells during long periods of growth (weeks). This poor mixing resulted in inhomogeneity in the cell suspension and invalidated calculation of cell multiplication rates in continuous-flow cultures. The development of such inhomogeneities was prevented by the combined use of large propeller blades for mixing and a growth-limiting substrate.
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