Abstract
In recent years, fluorescence light sheet-based imaging approaches have been developed to overcome the drawbacks of confocal microscopy, namely phototoxicity and photobleaching. Among these, lattice light sheet microscopy (LLSM) combines excellent 3D spatial resolution with fast temporal resolution and low phototoxicity at levels that cannot be matched by confocal microscopy. However, this potential has not yet been fully realized for investigations of individual cell behaviors and phenotypic changes in dense 3D bacterial biofilms.
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