Abstract
Introduction: Non fermentative Gram negative bacilli (NF GNB) are being increasingly isolated from patients admitted in hospitals. Most of these isolates are multidrug resistant (MDR). Methods: Over a period of one year, all clinical samples were processed and NF GNB was identified up to species level following conventional method. Antibiotic sensitivity test of the isolates were done following clinical and laboratory standard institute (CLSI) guidelines. Results: Out of 1498 clinical specimens, 320 (21.36%) isolates were identified as NF GNB. Maximum number of samples was blood, 90(28.12%), followed by pus, 84 (26.25%). Pseudomonas aeruginosa (P aeruginosa) was the commonest isolate, 192 (60%), followed by Pseudomonas species (P species), 58 (18.12%) & Acinetobacter baumannii (A baumannii), 46(14.37%). All isolates were MDR and were sensitive to polymyxin B, colistin and tigecycline. Conclusion: Multidrug resistant NF GNB was not uncommon in our hospital. All isolates were sensitive to polymyxin B, colistin and tigecycline. Indiscriminate use of antibiotics against these organisms should be avoided.
Highlights
Non fermentative Gram negative bacilli (NF GNB) are being increasingly isolated from patients admitted in hospitals
Antibiotic sensitivity test was performed by using modified Kirby – Bauer disk diffusion method following clinical and laboratory standard institute (CLSI) guidelines.[4]
The antibiotic disks used for this study were following – ceftazidime (10 μg), Amikacin (30 μg), Netilmycin (30 μg), ciprofloxacin (5μg), ticarcillin (75 μg), cefepime (30 μg), piperacillin/tazobactam (100/10μg), Imipenem (10μg), aztreonam (30 μg), colistin (10 μg), polymyxin B (50 μg) and tigecycline (15 μg)
Summary
Non fermentative Gram negative bacilli (NF GNB) are being increasingly isolated from patients admitted in hospitals. Most of these isolates are multidrug resistant (MDR). The non fermentative Gram negative bacilli (NF GNB) are aerobic and non spore forming microorganisms. NF GNB infection constitutes about one – fifth of all Gram negative bacilli infections These organisms can remain viable on medical devices and are resistant to many commonly used antibiotics. Rate of isolation of NF GNB from clinical specimens is increasing rapidly, very few laboratories in India identify these organisms routinely. Most of them are multidrug resistant (MDR).[1] Malini et al[2] from Karnataka, India observed 4.5 % infection rate of NF GNB in their hospital
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