Non-competitive inhibitioof ornithine decarboxylase by a phosphopeptide and phosphoamino acids

Abstract

In Tetrahymena pyriformis the cytosolic ornithine decarboxylase ( L-ornithine carboxy-lyase, EC 4.1.1.17) activity is considerably inhibited by the presence of polyamines in the growth medium, while the nuclear ornithine decarboxylase is only slightly affected. Experimental evidence suggests that the presence of putrescine and/or spermidine elicits the appearance of non-competitive inhibitors of ornithine decarboxylase. One pf the inhibitors has a molecular weight of 25 000 and properties of antizume. In addition, two other low molecular weight inhibitors are extracted, one which is a phosphoserine oligopeptide, and other which is phosphotyrosine. All inhibit non-competitively the homologous and heterologous ( Escherichia coli and rat liver) ornithine decarboxylases. Similarly, non-competitive inhibition was obtained when the commercially available phosphoamino acids wre tested against the already mentioned ornithine decarboxylases.