Noisy Juxtacellular Stimulation In Vivo Leads to Reliable Spiking and Reveals High-Frequency Coding in Single Neurons.

Abstract

Nanostimulation of single identified neurons in vivo can control spike frequency parametrically and, surprisingly, can even bias the animal's behavioral response. Here, we extend this stimulation protocol to time-dependent broadband noise stimulation in sensory and motor cortices of rat. In response to such stimuli, we found increased temporal spike-time reliability. The information transmission properties reveal, both experimentally and theoretically, that the neurons support high-frequency stimulation beyond the inverse membrane time. Generating a stimulus using the neuron's response properties, we could evoke prescribed spike times with high precision. Our work helps to establish a novel method for precise temporal control of single-cell spiking and provides a simplified biophysical description of single-neuron spiking under time-dependent in vivo-like stimulation.