Nociceptive, emotional and electrophysiological characterization of the chronic constriction injury model of experimental traumatic neuropathic pain in female Wistar Han rats.

Abstract

Event Abstract Back to Event Nociceptive, emotional and electrophysiological characterization of the chronic constriction injury model of experimental traumatic neuropathic pain in female Wistar Han rats. Diana M. Rodrigues1, 2*, Inês M. Laranjeira1, 2, João Barbosa1, 2, 3, Diana Amorim1, 2, Armando Almeida1, 2 and Filipa Pinto-Ribeiro1, 2 1 Life and Health Sciences Research Institute, School of Health Sciences, University of Minho, Portugal 2 PT Associate Laboratory ICVS/3B’s, Portugal 3 Polytechnic Institute of Viana do Castelo, Portugal Chronic neuropathic pain, affecting 7-10% of the population, is often accompanied by comorbid emotional impairments (depression/anxiety) that adversely affect pain by enhancing nociceptive symptomatology. This condition greatly reduces the quality of life of the patients, as it impairs physical, cognitive, emotional and social functioning, consequently interfering with treatment. Importantly, although chronic pain and emotional disorders are more prevalent in women, only a few publications focus on female animals. While the chronic constriction injury (CCI) model has been shown to mimic human neuropathies in terms of mechanical/thermal hyperalgesia, allodynia and spontaneous pain in both sexes, data on CCI-induced emotional impairments on female rats remains scarce. In this work, young adult female rats (Wistar Han) were divided into four groups (n=8 per group): gonadally intact females (SHAM/SHAM), ovariectomized females (SHAM/OVX), ovariectomized females with CCI (CCI/OVX) and gonadally intact females with CCI (CCI/SHAM) and the CCI model was induced by placing 4 chromic catgut constrictive ligatures in the common sciatic nerve. Nociceptive testing began before CCI surgery and was performed weekly throughout 5 weeks post-surgery after which the anxiodepressive-like behaviours were also assessed. In the postoperative period, CCI animals displayed visible gait abnormalities, limping and guarding the affected hind paw, and the development of mechanical (VonFrey test) and cold allodynia (acetone test) from week 1 onwards. While no differences were found between CCI and SHAM animals concerning anxiety-like (elevated plus maze and open field tests) and depressive-like (forced swimming and sucrose preference tests) behaviours, OVX females displayed anhedonic-like behaviour regardless of CCI surgery. Single cell electrophysiological recordings showed changes, in the neuronal activity of areas mediating nociceptive and emotional processing (the rostral ventromedial medulla and the amygdala), in accordance with our behavioural data. Keywords: Neuropathic pain (NP), Anxiety, Depression, Chronic constriction injury (CCI), Electrophysiology Conference: XVI Meeting of the Portuguese Society for Neuroscience (SPN2019), Lisboa, Portugal, 30 May - 1 Jun, 2019. Presentation Type: Poster presentation Topic: Sensory Processing Citation: Rodrigues DM, Laranjeira IM, Barbosa J, Amorim D, Almeida A and Pinto-Ribeiro F (2019). Nociceptive, emotional and electrophysiological characterization of the chronic constriction injury model of experimental traumatic neuropathic pain in female Wistar Han rats.. Front. Cell. Neurosci. Conference Abstract: XVI Meeting of the Portuguese Society for Neuroscience (SPN2019). doi: 10.3389/conf.fncel.2019.01.00016 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Feb 2019; Published Online: 27 Sep 2019. * Correspondence: Miss. Diana M Rodrigues, Life and Health Sciences Research Institute, School of Health Sciences, University of Minho, Braga, Portugal, diannamarina@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Diana M Rodrigues Inês M Laranjeira João Barbosa Diana Amorim Armando Almeida Filipa Pinto-Ribeiro Google Diana M Rodrigues Inês M Laranjeira João Barbosa Diana Amorim Armando Almeida Filipa Pinto-Ribeiro Google Scholar Diana M Rodrigues Inês M Laranjeira João Barbosa Diana Amorim Armando Almeida Filipa Pinto-Ribeiro PubMed Diana M Rodrigues Inês M Laranjeira João Barbosa Diana Amorim Armando Almeida Filipa Pinto-Ribeiro Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.