Abstract
Nitrogenase activity was observed in root-, stem- and leaf-tissue cultures of three genetic lines of Trifolium pratense infected with Rhizobium trifolii Rothamsted Strain 5, Stylosanthes gracilis with Rhizobium sp. Strain CB1552 and root-tissue cultures of carrot with Strains CB1552 and NGR99. Rhizobium (CB1552) previously associated with Stylosanthes tissues continued to show nitrogenase activity for 5 days after separation from the callus. Infected Trifolium callus differentiated to produce roots in 8 weeks on a medium free from NO 3 −, 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin, whereas the uninfected callus remained undifferentiated.
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