Nitrogen Regulates the Distribution of Antibiotic Resistance Genes in the Soil-Vegetable System.

Tingting Wang,Yanzheng Gao,Silu Sun,Yanxing Xu,Michael Gatheru Waigi,Xiaojie Hu,Emmanuel Stephen Odinga,Galina K Vasilyeva

doi:10.3389/fmicb.2022.848750

Abstract

The increasing antibiotic resistance genes (ARGs) in fertilizer-amended soils can potentially enter food chains through their transfer in a soil–vegetable system, thus, posing threats to human health. As nitrogen is an essential nutrient in agricultural production, the effect of nitrogen (in the forms NH4+-N and NO3−-N) on the distribution of ARGs (blaTEM-1, sul1, cmlA, str, and tetO) and a mobile genetic element (MGE; tnpA-4) in a soil–Chinese cabbage system was investigated. Not all the tested genes could transfer from soil to vegetable. For transferable ones (blaTEM-1, sul1, and tnpA-4), nitrogen application influenced their abundances in soil and vegetable but did not impact their distribution patterns (i.e., preference to either leaf or root tissues). For ARGs in soil, effects of nitrogen on their abundances varied over time, and the positive effect of NH4+-N was more significant than that of NO3−-N. The ARG accumulation to vegetables was affected by nitrogen application, and the nitrogen form was no longer a key influencing factor. In most cases, ARGs were found to prefer being enriched in roots, and nitrogen application may slightly affect their migration from root to leaf. The calculated estimated human intake values indicated that both children and adults could intake 106–107 copies of ARGs per day from Chinese cabbage consumption, and nitrogen application affected ARG intake to varying degrees. These results provided a new understanding of ARG distribution in vegetables under the agronomic measures such as nitrogen application, which may offer knowledge for healthy vegetable cultivation in future.

Highlights

Antibiotics play a vital role in protecting human health and promoting the development of animal husbandry
antibiotic resistance genes (ARGs) in soil could transfer to vegetable through two major ways and the rhizosphere is a hotspot for their transfer (Van Elsas et al, 2003; Wang et al, 2015; Yang et al, 2018)
Some other antibiotic resistant bacteria (ARB) cannot compete with the native plant endophytes and their growth would be largely suppressed, which might inhibit the propagation of their harboring ARGs (Xu et al, 2021)

Summary

Introduction

Antibiotics play a vital role in protecting human health and promoting the development of animal husbandry. Soil is known as an ARG reservoir, and ARGs in soil have been found to be transferred to vegetable endophytes (Yang et al, 2018), Nitrogen Regulates ARG Distribution which might enter into the human body during vegetable consumption, threatening human health (Zhu et al, 2017b). Under the application of organic fertilizers (such as manure), ARGs in the soil and the fertilizers would migrate into the plant, facilitating the ARG contamination (He et al, 2016). Zhou et al (2019) reported that the total ARG abundances in phyllosphere of rice was enhanced by ~20% and ~40% after a short term (within 1 month) of fertilization of pig manure collected from farms using reduced and standard antibiotic practices, respectively. Until now, studies have merely focused on their regulation on ARGs in the soil, and instead ignored their influence on the distribution and abundances of ARGs in soil–vegetable system

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Conclusion