Nitrogen limitation affects the sinking property of Microcystis by promoting carbohydrate accumulation

Abstract

Nitrogen limitation has been proven to inhibit Microcystis proliferation, and the significant decline in Microcystis blooms in late summer or autumn has been considered to be related to the nitrogen depletion in water. Sinking loss is another factor that influences the dynamics of cyanobacteria in lakes. However, to date, it is still unclear how the sinking property of Microcystis responds to nitrogen availability. Our results suggest that nitrogen limitation would directly influence sinking property of Microcystis, through a significant increase in the specific density of cells. In the short term, carbohydrate accumulation was mainly responsible for the high specific density, showing a high correlation among the NO3−-N concentration, specific density and carbohydrate content. Furthermore, carbohydrates could rapidly accumulate after one light/dark cycle, which was mainly due to the reduction in carbohydrate consumption in the darkness under nitrogen limitation. Under nitrogen-light coupling conditions, the specific density ranged from 1.060 to 1.068, except for the treatment with high-nitrogen plus low-light, which showed the value of 1.032. More importantly, when coupled with low nitrogen, the low light did not decrease the carbohydrate content and the specific density, which implied that the sinking cells could not migrate back to the surface. Accordingly, a hypothesis was proposed that the carbohydrate accumulation induced by low nitrogen availability caused an increase in specific density, which invalidates the buoyancy regulation, and cells sink continually out of the water column. This study explores a new understanding on the disappearance mechanisms of Microcystis blooms in the late summer and fall.