Abstract
Summary Leaves bearing bacteria-filled nodules from seven Pavetta and two Psychotria species were tested for nitrogenase activity by the acetylene reduction method for up to 24 hours with negative results. Four of the Pavetta species and the two Psychotria species used in the acetylene reduction tests were also grown in a nitrogen-free culture solution for 75 days. The plants developed severe symptoms of nitrogen deficiency and no increase in their nitrogen content could be detected during the experimental period.
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