Nitrogen addition exerts a stronger effect than elevated temperature on soil available nitrogen and relation to soil microbial properties in the rhizosphere of Camellia sinensis L. seedlings.

Abstract

As the global climate changes, elevated atmospheric temperature and nitrogen (N) deposition co-occur in natural ecosystems, which affects rhizosphere soil nutrient by altering allocation of roots and its availability to soil microorganism. Elevated temperature in combination with N deposition is expected to affect soil available N and its relation to microbial properties, but this issue has not been extensively examined. Here, we investigated soil available N and its relation to microbial properties in rhizosphere of Camellia sinensis L. seedlings exposed to elevated temperature using a passive warming device in combination with N-added soil. Elevated temperature did not significantly affect soil pH, total organic carbon (TOC), total nitrogen (TN), the ratio of carbon and nitrogen (C:N ratio), total phosphorus (TP), available N ((N in ammonium (NH4+-N) and N in nitrate (NO3--N)) (NH4+-N + NO3--N)/TN, α-glucosidase (αG), β-glucosidase (βG), cellobiohydrolase (CBH), N-acetyl-glucosaminidase (NAG), and phenol oxidase (PPO) activities, while significantly stimulated root total length of tea seedlings (3.9%), root dry biomass (10.2%), soil microbial biomass carbon (MBC) (7.4%), microbial biomass nitrogen (MBN) (8.6%), and acid phosphatase (ACP) (8.8%). While N addition significantly (p < 0.05) stimulated root dry biomass of tea seedlings (14.1%), root total length (6.2%), root average diameter (6.7%), soil TN, available N, (NH4+-N + NO3--N)/TN, and MBN under elevated temperature. Soil aG, βG, CBH, and ACP activity increase significantly (p < 0.05) under elevated temperature + N relative to elevated temperature alone. Generally, N addition led to increased available nitrogen and microbial properties in rhizosphere soil of tea seedlings exposed to elevated temperature by stimulating root properties, soil nitrogen, microbial biomass N, and enzyme activity. Redundancy analysis and Pearson correlation analysis suggested that N addition lead to higher correlation between soil available N and microbial properties exposed to elevated temperature. Our results indicated nitrogen addition exerts a stronger effect than elevated temperature on soil fertility and microbiological cycle in the rhizosphere of Camellia sinensis L. seedlings. The conclusion helps us understand the response mechanism of soil rhizosphere microenvironment to N deposition under global warming scenarios.