Abstract

Nitric oxide-mediated drought stress tolerance via improvement crop yield, antioxidants, membrane integrity and reducing the oxidative stress of two faba bean cultivars

Highlights

  • Plants in a natural environment exposed to pathogens attacks such as bacteria, fungi, and viruses as well as abiotic environmental factors as drought, salt, floods, heat, cold and heavy metals that can affect the average growth, development, and production, affect the global food security [1]

  • Non- enzymatic antioxidants Ascorbic acid (ASA) and reduced glutathione (GSH): 0.5 g fresh leaves were mixed in 5% trichloroacetic acid, centrifuged at 11,500 × g for 15 min at 4 °C, and the supernatant was utilized for quantification of ASA and GSH by protocols of Jagota and Dani [16], respectively

  • Nitric oxide (NO) curtailed the reduction of crop yield of the drought-stressed plant (seeds yield/plant, (Figure 1F, f) and enhanced the yield of well-watered plants of Giza 843

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Summary

Introduction

Plants in a natural environment exposed to pathogens attacks such as bacteria, fungi, and viruses as well as abiotic environmental factors as drought, salt, floods, heat, cold and heavy metals that can affect the average growth, development, and production, affect the global food security [1]. The amelioration of oxidative burst and enhanced resistance to environmental stresses is often associated with the equilibrium of the antioxidative system and ROS production. These responses may be activated in plants via application protecting substances to produce vigor plants that can withstand harsh conditions. The co-workers tended to soak plants in the selected protective substance, NO, with a proper concentration picked up from a preliminary experiment (data not shown) to elucidate the antioxidative response of drought faba bean plants to NOdonor (sodium nitroprusside), and they reflect on plant growth as well as crop yield production. We have conducted this research to confirm the nitric oxide-mediated drought stress tolerance via improvement crop yield, antioxidants, membrane integrity, and reducing the oxidative stress of two faba bean cultivars

Experimental setup
Plant growth parameters Root and shoot lengths were measured and expressed in cm
Reactive oxygen species
Oxidative stress markers Electrolyte leakage (EL) was estimated as given by
Non- enzymatic antioxidants
Metabolites Proline content was elicited according to
Enzymatic antioxidants
Results and discussion
Conclusion
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