Evaluation of Gene Expression for Orobanche Tolerance in Faba bean (Vicia faba L.)

Abstract

Two faba bean cultivars were studied for Orobanche (Orobanche crenata, forsk) tolerance. A greenhouse experiment was conducted using two Egyptian faba bean (V. faba L.) cultivars infected by Orobanche crenata in a pot trial. Results of the control plants (without Orobanche infection) and infected plants (with or without Orobanche attachment) proved that Giza 3 was the susceptible cultivar and G.843 was tolerant which had the lowest Orobanche tubercles per plant. Spectrophotometer was used to assess total protein, peroxidase and polyphenoloxidase activity extracted from faba bean roots and shoots. SDS-PAGE of roots and shoots extracts and isozymes-electrophoreses were used to assess the genetic expression of the two faba bean cultivars according to their banding patterns.