Abstract
Growth, nitrate reductase activity (NRA; EC 1.6.6.1), nitrate content and soluble protein concentration were studied in pea ( Pisum sativum cv. Lincoln) plants supplied with different NOf concentrations and inoculated with selected Rhizobiwn leguminosarum strains. Non-inoculated plants grown in the same nutrient medium served as controls. NRA was determined by an in vivo (−NO 3 −) assay. Differences in tissue NRA were mainly related to NO 3 − concentration in the growth medium; however, nodulation markedly influenced the amounts of NRA in different plant organs, especially roots. The concentration of NO 3 − in leaves, stems and roots increased markedly with NO 3 − dose. Although the differences in the NO 3 − accumulation were mainly ascribed to NO 3 − treatment, nodulation also affected the NO 3 − content of tissues, especially of roots and stems.
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