NITRATE ACCUMULATION IN SPINACH AS INFLUENCED BY SULFUR AND PHOSPHORUS APPLICATION UNDER INCREASING NITROGEN LEVELS

Abstract

Nitrate (NO3) accumulation by spinach was studied under increasing nitrogen (N) levels (60, 120 and 240 kg N ha−1) along with sulfur (45 kg S ha−1) and phosphorus (P; 90 kg P2O5 ha−1) application. Plants were harvested at 50 and 65 days after sowing. Plant samples were analyzed for NO3-N and total N, P, S, potassium (K), calcium (Ca), and magnesium (Mg). Radio assay of 35S was done to estimate percent sulfur derived from fertilizer and percent fertilizer sulfur utilization. Spinach maintained a very high level of NO3-N in its tissue throughout the growing period. NO3-N was increased with increasing nitrogen level and was reduced with phosphorus and sulfur application and also with advancement in growth. Total N, P, S, K, Ca and Mg uptake were increased with increasing nitrogen levels as well as with application of sulfur and phosphorus. Sulfur application caused increase in percent sulfur derived from fertilizer and percent utilization of fertilizer sulfur.