Abstract
Nickel uptake system was investigated with a wild-type cell of Rhodopseudomonas capsulata and two mutants lacking uptake hydrogenase (Hup-). Wild type cells grown photoheterotrophically incorporated 63Ni2+ by a high affinity system. The uptake system had a pH of 7.0 and a temperature optimum of 28°C. Both Mg2+ and Co2+ ions severely repressed the uptake of Ni2+. Nickel transport was also inhibited by metabolic inhibitors including cyanide, azide, 2,4-dinitrophenol and m-chlorophenyl carbonylcyanidehydrazone. These data imply that Ni2+ uptake system occurs by the energy-linked system for Mg2+ transport. The intracellular distribution of 63Ni2+ in Hup- cells showed the same pattern as that of wild-type cells, indicate that the Hup- strains have no deficiency in Ni2+ transport.
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