Abstract
Influx of Ca 2+ induced by the synergistic action of glucagon plus vasopressin in the perfused rat liver was progressively inhibited by infusing increasing concentrations of Ni 2+ to the perfusion medium. The onset of Ca 2+ influx following vasopressin administration was delayed and inhibition occurred of both the initial rate of Ca 2+ influx as well as the total amount of Ca 2+ taken up by the liver. Inhibition of the Ca 2+ influx rate was almost maximal at approximately 500 μM Ni 2+; half-maximal inhibition occurred at less than 250 μM. Added Ni 2+ also delayed the onset of the early transient bile flow peak. In addition, the duration of the transient peak in bile flow was prolonged by approximately 2 min by all concentrations of Ni 2+ between 25–500 μM, the greatest amount of bile being released in the presence of 250 μM Ni 2+. Concentrations of Ni 2+ at 100 μM and above also inhibit the decrease in bile flow to below baseline levels. The data identify a multiple role for Ca 2+ mobilisation in bile flow.
Published Version
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