Abstract
Dendrobium lituiflorum Lindl. is endemic, rare and threatened Vietnam orchids. Therefore, in order to meet the demand for a large scale production, a good propagation technique of high quality D. lituiflorum transplants is essential. In this study, the effects of sucrose concentration (0, 5, 10, 15, 20 g/L), mineral medium strength (MS, ½MS, ¼MS) on the development of shoots, roots, ex vitro planlets in shoot ventilation culture of D. lituiflorum were evaluated. The highest number of roots and ex vitro plantlet survival was observed on ½MS and ¼MS medium. Increasing numbers of shoot and root were observed in high sucrose-containing medium. However the highest survival rate was observed in ½MS medium supplemented with 10g/L sucrose (70%). This study suggests that sucrose concentration, mineral medium strength is key factors to increase plantlet survival during acclimatization.
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