Abstract
Hibiscus sagittifolius is not only a beautiful flower, but also species having high medicinal value. This study is aimed to complete the process of in vitro propagation of H.sagittifolius from seeds and nodes. The results show that the percentage of uninfected and survived samples was 71.99% when the seeds and nodes were sterilized with 15% NaClO solution for 15 min. The seed germination rate was improved when seed was pre-treated by immersion in 20 mg/L GA3 for 120 min before being sterilized. The MS medium supplemented with 2.0 mg/L BA, 30.0 g/L sucrose, 100.0 mL/L coconut water, pH 5.8 was suitable for multiplication of in vitro shoots. After 45 days of culture, the number of shoots obtained was 6.6 shoots per sample and the shoots’ average height was 3.28 cm. Afterwards, the in vitro shoots were inoculated on MS medium supplemented with 1 mg/L NAA for rooting. After 45 days of culture, the number of roots per shoot was 33.6 with an average length of 13 cm.
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