Abstract
From leaves and terminal branches of Melaleuca alternifolia grown in Tien Giang province, the Melaleuca alternifolia oil - Tea Tree oil (TTO) was extracted by steam distillation method. Extraction process parameters were studied including distillation time and temperature and material preservation time. Chemical ingredients of TTO were identified by modern analytical method GC-MS. Collected TTO was used as the main antibacterial ingredient in mouthwash product. The results showed that, the prime conditions for distillation were at 100oC and within 100 minutes. The average distillation productivity of TTO was 4.91% (wt/wt) with the main components including Terpinen-4-ol (36%), γ-Terpinene (17.8%) and 1,8-Cineole (10%) which reached the ISO 4730:2004 standard. The obtained mouthwash product possessed the same antibiotic activity as commercial product against Staphylococcus aureus and Pseudomonas aeruginosa. These results contributed to find a new application of TTO which would increase its economic value in Viet Nam.
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