Abstract
Abstract: The synthesis and application of a novel analytical reagent, [TREN 3 (2 Quinoline)], was detailed for the precise determination of nickel (II) concentrations in biological samples and plant materials. The analysis involved extracting the nickel(II)-TREN3(2Quinoline) complex from chloroform before injection into the instrument. To ensure accuracy and various parameters such as pH, pulse amplitude, scan rate, and choice of solvent were meticulously optimized. A calibration curve constructed in the concentration range from 0.05 to 42 µg mL-1 under pH 4.0, conditions using an acetate buffer, exhibiting a high correlation coefficient of 0.992. The study also thoroughly investigated potential interference from diverse ions, Validation of the proposed method was conducted using Certified Reference Materials provided by the National Institute of Standard Technology, affirming its accuracy and reliability. Furthermore, the reagent underwent characterization through techniques including infrared spectroscopy (IR), mass spectrometry (Mass), and elemental analysis, bolstering confidence in its suitability for analytical purposes.
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